Trichoderma asperellum, a potential biological control agent of Stemphylium vesicarium, on onion (Allium cepa L.)

Diego Helman Zapata-Sarmiento, Elibeth Fabiola Palacios-Pala, Aida Araceli Rodríguez-Hernández, Diana Laura Medina Melchor, Mario Rodríguez-Monroy, Gabriela Sepúlveda-Jiménez

Resultado de la investigación: Contribución a una revistaArtículo

Resumen

Leaf blight disease caused by Stemphylium vesicarium affects onion production in several countries; in Mexico the disease has scarcely been documented. In this study, we identified S. vesicarium in onion plants from Mexico with symptoms of leaf blight, and evaluated the potential of Trichoderma asperellum as a biological control agent against S. vesicarium. The in vitro antifungal activity of T. asperellum isolates from cultures of mango (Tm), tomato (Tt) and onion (To) were evaluated against S. vesicarium. In dual culture assays, the three T. asperellum isolates inhibited between 54 and 61% of S. vesicarium mycelial growth. Using the ‘poisoned food technique’ we showed that the cell-free culture filtrate of T. asperellum isolate To inhibited growth of S. vesicarium by 61% and reduced conidia production by 75% compared with controls; culture filtrates from isolates Tt and Tm were also inhibitory but less so than isolate To. When onion plants were inoculated with T. asperellum isolate To the severity of disease symptoms caused by S. vesicarium were reduced compared with control plants. Plants infected with S. vesicarium had 62 and 34% less chlorophyll and carotenoids, respectively than control plants; prior inoculation with T. asperellum ameliorated this reduction in chlorophyll and carotenoid content in S. vesicarium-infected plants; the chlorophyll content was reduced by only 38% and the carotenoids content was similar to that found in control plants. The levels of phenolic compounds in plant tissues changed depending on experimental treatment; inoculation with T. asperellum increased the quantity of phenolic compounds by 97.6% compared with the control but only in onion bulbs. In contrast, inoculation with S. vesicarium increased the quantity of phenolic compounds in leaves and roots by 50 and 88.9%, respectively, compared with the control. These data suggest that T. asperellum isolate To has potential for biological control of S. vesicarium in onion crops.

Idioma originalInglés
Número de artículo104105
PublicaciónBiological Control
Volumen140
DOI
EstadoPublicada - ene 2020

Huella dactilar

Stemphylium vesicarium
Trichoderma asperellum
Allium cepa
onions
biological control agents
phenolic compounds
carotenoids
leaf blight
culture filtrates
chlorophyll
signs and symptoms (plants)
Mexico
tomatoes
mangoes
bulbs
disease severity
conidia
plant tissues

Citar esto

Zapata-Sarmiento, D. H., Palacios-Pala, E. F., Rodríguez-Hernández, A. A., Medina Melchor, D. L., Rodríguez-Monroy, M., & Sepúlveda-Jiménez, G. (2020). Trichoderma asperellum, a potential biological control agent of Stemphylium vesicarium, on onion (Allium cepa L.). Biological Control, 140, [104105]. https://doi.org/10.1016/j.biocontrol.2019.104105
Zapata-Sarmiento, Diego Helman ; Palacios-Pala, Elibeth Fabiola ; Rodríguez-Hernández, Aida Araceli ; Medina Melchor, Diana Laura ; Rodríguez-Monroy, Mario ; Sepúlveda-Jiménez, Gabriela. / Trichoderma asperellum, a potential biological control agent of Stemphylium vesicarium, on onion (Allium cepa L.). En: Biological Control. 2020 ; Vol. 140.
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title = "Trichoderma asperellum, a potential biological control agent of Stemphylium vesicarium, on onion (Allium cepa L.)",
abstract = "Leaf blight disease caused by Stemphylium vesicarium affects onion production in several countries; in Mexico the disease has scarcely been documented. In this study, we identified S. vesicarium in onion plants from Mexico with symptoms of leaf blight, and evaluated the potential of Trichoderma asperellum as a biological control agent against S. vesicarium. The in vitro antifungal activity of T. asperellum isolates from cultures of mango (Tm), tomato (Tt) and onion (To) were evaluated against S. vesicarium. In dual culture assays, the three T. asperellum isolates inhibited between 54 and 61{\%} of S. vesicarium mycelial growth. Using the ‘poisoned food technique’ we showed that the cell-free culture filtrate of T. asperellum isolate To inhibited growth of S. vesicarium by 61{\%} and reduced conidia production by 75{\%} compared with controls; culture filtrates from isolates Tt and Tm were also inhibitory but less so than isolate To. When onion plants were inoculated with T. asperellum isolate To the severity of disease symptoms caused by S. vesicarium were reduced compared with control plants. Plants infected with S. vesicarium had 62 and 34{\%} less chlorophyll and carotenoids, respectively than control plants; prior inoculation with T. asperellum ameliorated this reduction in chlorophyll and carotenoid content in S. vesicarium-infected plants; the chlorophyll content was reduced by only 38{\%} and the carotenoids content was similar to that found in control plants. The levels of phenolic compounds in plant tissues changed depending on experimental treatment; inoculation with T. asperellum increased the quantity of phenolic compounds by 97.6{\%} compared with the control but only in onion bulbs. In contrast, inoculation with S. vesicarium increased the quantity of phenolic compounds in leaves and roots by 50 and 88.9{\%}, respectively, compared with the control. These data suggest that T. asperellum isolate To has potential for biological control of S. vesicarium in onion crops.",
keywords = "Beneficial fungi, Carotenoid, Chlorophyll, Defense response, Leaf blight, Phenolic compounds",
author = "Zapata-Sarmiento, {Diego Helman} and Palacios-Pala, {Elibeth Fabiola} and Rodr{\'i}guez-Hern{\'a}ndez, {Aida Araceli} and {Medina Melchor}, {Diana Laura} and Mario Rodr{\'i}guez-Monroy and Gabriela Sep{\'u}lveda-Jim{\'e}nez",
year = "2020",
month = "1",
doi = "10.1016/j.biocontrol.2019.104105",
language = "Ingl{\'e}s",
volume = "140",
journal = "Biological Control",
issn = "1049-9644",
publisher = "Academic Press Inc.",

}

Zapata-Sarmiento, DH, Palacios-Pala, EF, Rodríguez-Hernández, AA, Medina Melchor, DL, Rodríguez-Monroy, M & Sepúlveda-Jiménez, G 2020, 'Trichoderma asperellum, a potential biological control agent of Stemphylium vesicarium, on onion (Allium cepa L.)', Biological Control, vol. 140, 104105. https://doi.org/10.1016/j.biocontrol.2019.104105

Trichoderma asperellum, a potential biological control agent of Stemphylium vesicarium, on onion (Allium cepa L.). / Zapata-Sarmiento, Diego Helman; Palacios-Pala, Elibeth Fabiola; Rodríguez-Hernández, Aida Araceli; Medina Melchor, Diana Laura; Rodríguez-Monroy, Mario; Sepúlveda-Jiménez, Gabriela.

En: Biological Control, Vol. 140, 104105, 01.2020.

Resultado de la investigación: Contribución a una revistaArtículo

TY - JOUR

T1 - Trichoderma asperellum, a potential biological control agent of Stemphylium vesicarium, on onion (Allium cepa L.)

AU - Zapata-Sarmiento, Diego Helman

AU - Palacios-Pala, Elibeth Fabiola

AU - Rodríguez-Hernández, Aida Araceli

AU - Medina Melchor, Diana Laura

AU - Rodríguez-Monroy, Mario

AU - Sepúlveda-Jiménez, Gabriela

PY - 2020/1

Y1 - 2020/1

N2 - Leaf blight disease caused by Stemphylium vesicarium affects onion production in several countries; in Mexico the disease has scarcely been documented. In this study, we identified S. vesicarium in onion plants from Mexico with symptoms of leaf blight, and evaluated the potential of Trichoderma asperellum as a biological control agent against S. vesicarium. The in vitro antifungal activity of T. asperellum isolates from cultures of mango (Tm), tomato (Tt) and onion (To) were evaluated against S. vesicarium. In dual culture assays, the three T. asperellum isolates inhibited between 54 and 61% of S. vesicarium mycelial growth. Using the ‘poisoned food technique’ we showed that the cell-free culture filtrate of T. asperellum isolate To inhibited growth of S. vesicarium by 61% and reduced conidia production by 75% compared with controls; culture filtrates from isolates Tt and Tm were also inhibitory but less so than isolate To. When onion plants were inoculated with T. asperellum isolate To the severity of disease symptoms caused by S. vesicarium were reduced compared with control plants. Plants infected with S. vesicarium had 62 and 34% less chlorophyll and carotenoids, respectively than control plants; prior inoculation with T. asperellum ameliorated this reduction in chlorophyll and carotenoid content in S. vesicarium-infected plants; the chlorophyll content was reduced by only 38% and the carotenoids content was similar to that found in control plants. The levels of phenolic compounds in plant tissues changed depending on experimental treatment; inoculation with T. asperellum increased the quantity of phenolic compounds by 97.6% compared with the control but only in onion bulbs. In contrast, inoculation with S. vesicarium increased the quantity of phenolic compounds in leaves and roots by 50 and 88.9%, respectively, compared with the control. These data suggest that T. asperellum isolate To has potential for biological control of S. vesicarium in onion crops.

AB - Leaf blight disease caused by Stemphylium vesicarium affects onion production in several countries; in Mexico the disease has scarcely been documented. In this study, we identified S. vesicarium in onion plants from Mexico with symptoms of leaf blight, and evaluated the potential of Trichoderma asperellum as a biological control agent against S. vesicarium. The in vitro antifungal activity of T. asperellum isolates from cultures of mango (Tm), tomato (Tt) and onion (To) were evaluated against S. vesicarium. In dual culture assays, the three T. asperellum isolates inhibited between 54 and 61% of S. vesicarium mycelial growth. Using the ‘poisoned food technique’ we showed that the cell-free culture filtrate of T. asperellum isolate To inhibited growth of S. vesicarium by 61% and reduced conidia production by 75% compared with controls; culture filtrates from isolates Tt and Tm were also inhibitory but less so than isolate To. When onion plants were inoculated with T. asperellum isolate To the severity of disease symptoms caused by S. vesicarium were reduced compared with control plants. Plants infected with S. vesicarium had 62 and 34% less chlorophyll and carotenoids, respectively than control plants; prior inoculation with T. asperellum ameliorated this reduction in chlorophyll and carotenoid content in S. vesicarium-infected plants; the chlorophyll content was reduced by only 38% and the carotenoids content was similar to that found in control plants. The levels of phenolic compounds in plant tissues changed depending on experimental treatment; inoculation with T. asperellum increased the quantity of phenolic compounds by 97.6% compared with the control but only in onion bulbs. In contrast, inoculation with S. vesicarium increased the quantity of phenolic compounds in leaves and roots by 50 and 88.9%, respectively, compared with the control. These data suggest that T. asperellum isolate To has potential for biological control of S. vesicarium in onion crops.

KW - Beneficial fungi

KW - Carotenoid

KW - Chlorophyll

KW - Defense response

KW - Leaf blight

KW - Phenolic compounds

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U2 - 10.1016/j.biocontrol.2019.104105

DO - 10.1016/j.biocontrol.2019.104105

M3 - Artículo

AN - SCOPUS:85073521324

VL - 140

JO - Biological Control

JF - Biological Control

SN - 1049-9644

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