T-cell suppression and selective in vivo activation of TH2 subpopulation by the Entamoeba histolytica 220-kilodalton lectin

P. Talamas-Rohana, M. A. Schlie-Guzman, V. I. Hernandez-Ramirez, J. L. Rosales- Encina

Resultado de la investigación: Contribución a una revistaArtículo

25 Citas (Scopus)

Resumen

A 220-kDa surface protein (L220) with lectin activity from Entamoeba histolytica trophozoites has been characterized previously (J. L. Rosales- Encina, I. Meza, A. Lopez-de-Leon, P. Talamas-Rohana, and M. Rojkind, J. Infect. Dis. 156:790-797, 1987). This molecule is involved in the adhesion process (I. Meza, F. Cazares, J. L. Rosales-Encina, P. Talamas-Rohana, and M. Rojkind, J. Infect. Dis. 156:798-805, 1987) and is very immunogenic. In this work, we studied both the humoral and the cellular immune responses to L220. We compared L220 with L220-derived components, such as a fusion peptide (M- 11) and chemically obtained peptides (by treating the 220-kDa molecule with N-chlorosuccinimide-urea). Spleen cells from L220-immunized mice were unable to proliferate in vitro when stimulated with the protein. However, a proliferative response was obtained when mice were immunized with the L220- derived fusion peptide or the cleaved lectin. To find out if there was a correlation between the observed responses and TH1 or TH2 activation, we analyzed patterns of cytokine secretion (interleukin-2 [IL-2], IL-4, IL-10, and gamma interferon). Cells from mice immunized with peptides that induced cell proliferation (100, 80, and 47 kDa) with the peptides (P < 0.01) and with the intact molecule secreted IL-2 and gamma interferon, showing a TH1- subset pattern. Conversely, cells from mice immunized with the intact 220- kDa molecule secreted IL-4 and IL-10, typical of a TH2 subpopulation; however, antibodies from each group recognized the 220-kDa molecule as determined by Western blotting (immunoblotting). These results suggest that various epitopes in the 220-kDa molecule generate different response patterns, suppressing or activating T-cell responses.

Idioma originalInglés
Páginas (desde-hasta)3953-3958
Número de páginas6
PublicaciónInfection and immunity
Volumen63
N.º10
EstadoPublicada - 1 ene 1995

Huella dactilar

Entamoeba histolytica
Lectins
T-Lymphocytes
Interleukin-4
Interleukin-10
Peptides
Interferon-gamma
Interleukin-2
Rosales
Western Blotting
Trophozoites
Humoral Immunity
Cellular Immunity
Urea
Epitopes
Membrane Proteins
Spleen
Cell Proliferation
Cytokines
Antibodies

Citar esto

Talamas-Rohana, P. ; Schlie-Guzman, M. A. ; Hernandez-Ramirez, V. I. ; Rosales- Encina, J. L. / T-cell suppression and selective in vivo activation of TH2 subpopulation by the Entamoeba histolytica 220-kilodalton lectin. En: Infection and immunity. 1995 ; Vol. 63, N.º 10. pp. 3953-3958.
@article{80bb1cd1d34a4acda6e268c99c095bec,
title = "T-cell suppression and selective in vivo activation of TH2 subpopulation by the Entamoeba histolytica 220-kilodalton lectin",
abstract = "A 220-kDa surface protein (L220) with lectin activity from Entamoeba histolytica trophozoites has been characterized previously (J. L. Rosales- Encina, I. Meza, A. Lopez-de-Leon, P. Talamas-Rohana, and M. Rojkind, J. Infect. Dis. 156:790-797, 1987). This molecule is involved in the adhesion process (I. Meza, F. Cazares, J. L. Rosales-Encina, P. Talamas-Rohana, and M. Rojkind, J. Infect. Dis. 156:798-805, 1987) and is very immunogenic. In this work, we studied both the humoral and the cellular immune responses to L220. We compared L220 with L220-derived components, such as a fusion peptide (M- 11) and chemically obtained peptides (by treating the 220-kDa molecule with N-chlorosuccinimide-urea). Spleen cells from L220-immunized mice were unable to proliferate in vitro when stimulated with the protein. However, a proliferative response was obtained when mice were immunized with the L220- derived fusion peptide or the cleaved lectin. To find out if there was a correlation between the observed responses and TH1 or TH2 activation, we analyzed patterns of cytokine secretion (interleukin-2 [IL-2], IL-4, IL-10, and gamma interferon). Cells from mice immunized with peptides that induced cell proliferation (100, 80, and 47 kDa) with the peptides (P < 0.01) and with the intact molecule secreted IL-2 and gamma interferon, showing a TH1- subset pattern. Conversely, cells from mice immunized with the intact 220- kDa molecule secreted IL-4 and IL-10, typical of a TH2 subpopulation; however, antibodies from each group recognized the 220-kDa molecule as determined by Western blotting (immunoblotting). These results suggest that various epitopes in the 220-kDa molecule generate different response patterns, suppressing or activating T-cell responses.",
author = "P. Talamas-Rohana and Schlie-Guzman, {M. A.} and Hernandez-Ramirez, {V. I.} and {Rosales- Encina}, {J. L.}",
year = "1995",
month = "1",
day = "1",
language = "Ingl{\'e}s",
volume = "63",
pages = "3953--3958",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "10",

}

Talamas-Rohana, P, Schlie-Guzman, MA, Hernandez-Ramirez, VI & Rosales- Encina, JL 1995, 'T-cell suppression and selective in vivo activation of TH2 subpopulation by the Entamoeba histolytica 220-kilodalton lectin', Infection and immunity, vol. 63, n.º 10, pp. 3953-3958.

T-cell suppression and selective in vivo activation of TH2 subpopulation by the Entamoeba histolytica 220-kilodalton lectin. / Talamas-Rohana, P.; Schlie-Guzman, M. A.; Hernandez-Ramirez, V. I.; Rosales- Encina, J. L.

En: Infection and immunity, Vol. 63, N.º 10, 01.01.1995, p. 3953-3958.

Resultado de la investigación: Contribución a una revistaArtículo

TY - JOUR

T1 - T-cell suppression and selective in vivo activation of TH2 subpopulation by the Entamoeba histolytica 220-kilodalton lectin

AU - Talamas-Rohana, P.

AU - Schlie-Guzman, M. A.

AU - Hernandez-Ramirez, V. I.

AU - Rosales- Encina, J. L.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - A 220-kDa surface protein (L220) with lectin activity from Entamoeba histolytica trophozoites has been characterized previously (J. L. Rosales- Encina, I. Meza, A. Lopez-de-Leon, P. Talamas-Rohana, and M. Rojkind, J. Infect. Dis. 156:790-797, 1987). This molecule is involved in the adhesion process (I. Meza, F. Cazares, J. L. Rosales-Encina, P. Talamas-Rohana, and M. Rojkind, J. Infect. Dis. 156:798-805, 1987) and is very immunogenic. In this work, we studied both the humoral and the cellular immune responses to L220. We compared L220 with L220-derived components, such as a fusion peptide (M- 11) and chemically obtained peptides (by treating the 220-kDa molecule with N-chlorosuccinimide-urea). Spleen cells from L220-immunized mice were unable to proliferate in vitro when stimulated with the protein. However, a proliferative response was obtained when mice were immunized with the L220- derived fusion peptide or the cleaved lectin. To find out if there was a correlation between the observed responses and TH1 or TH2 activation, we analyzed patterns of cytokine secretion (interleukin-2 [IL-2], IL-4, IL-10, and gamma interferon). Cells from mice immunized with peptides that induced cell proliferation (100, 80, and 47 kDa) with the peptides (P < 0.01) and with the intact molecule secreted IL-2 and gamma interferon, showing a TH1- subset pattern. Conversely, cells from mice immunized with the intact 220- kDa molecule secreted IL-4 and IL-10, typical of a TH2 subpopulation; however, antibodies from each group recognized the 220-kDa molecule as determined by Western blotting (immunoblotting). These results suggest that various epitopes in the 220-kDa molecule generate different response patterns, suppressing or activating T-cell responses.

AB - A 220-kDa surface protein (L220) with lectin activity from Entamoeba histolytica trophozoites has been characterized previously (J. L. Rosales- Encina, I. Meza, A. Lopez-de-Leon, P. Talamas-Rohana, and M. Rojkind, J. Infect. Dis. 156:790-797, 1987). This molecule is involved in the adhesion process (I. Meza, F. Cazares, J. L. Rosales-Encina, P. Talamas-Rohana, and M. Rojkind, J. Infect. Dis. 156:798-805, 1987) and is very immunogenic. In this work, we studied both the humoral and the cellular immune responses to L220. We compared L220 with L220-derived components, such as a fusion peptide (M- 11) and chemically obtained peptides (by treating the 220-kDa molecule with N-chlorosuccinimide-urea). Spleen cells from L220-immunized mice were unable to proliferate in vitro when stimulated with the protein. However, a proliferative response was obtained when mice were immunized with the L220- derived fusion peptide or the cleaved lectin. To find out if there was a correlation between the observed responses and TH1 or TH2 activation, we analyzed patterns of cytokine secretion (interleukin-2 [IL-2], IL-4, IL-10, and gamma interferon). Cells from mice immunized with peptides that induced cell proliferation (100, 80, and 47 kDa) with the peptides (P < 0.01) and with the intact molecule secreted IL-2 and gamma interferon, showing a TH1- subset pattern. Conversely, cells from mice immunized with the intact 220- kDa molecule secreted IL-4 and IL-10, typical of a TH2 subpopulation; however, antibodies from each group recognized the 220-kDa molecule as determined by Western blotting (immunoblotting). These results suggest that various epitopes in the 220-kDa molecule generate different response patterns, suppressing or activating T-cell responses.

UR - http://www.scopus.com/inward/record.url?scp=0029164352&partnerID=8YFLogxK

M3 - Artículo

C2 - 7558304

AN - SCOPUS:0029164352

VL - 63

SP - 3953

EP - 3958

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 10

ER -