Production of plants resistant to Alternaria carthami via organogenesis and somatic embryogenesis of safflower cv. NARI-6 treated with fungal culture filtrates

J. Vijaya Kumar, B. D. Ranjitha Kumari, G. Sujatha, Enrique Castaño De la serna

Resultado de la investigación: Contribución a una revistaArtículo

15 Citas (Scopus)

Resumen

The present study describes a system for efficient plant regeneration via organogenesis and somatic embryogenesis of safflower (Carthamus tinctorius L.) cv. NARI-6 in fungal culture filtrates (FCF)-treated cultures. FCF was prepared by culturing Alternaria carthami fungal mycelia in selection medium for host-specific toxin production. Cotyledon explants cultured on callus induction medium with different levels of FCF (10-50%) produced embryogenic callus. In organogenesis, 42.2% microshoots formed directly from embryogenic callus tissues in plant regeneration medium with 40% FCF. Isolated embryogenic callus cultured on embryo induction medium containing 40% FCF induced 50.2% somatic embryogenesis. Embryo germination percentage was decreased from 64.5 to 28 in embryo maturation medium containing 40% FCF. However, nine plantlets from organogenesis and 24 plantlets from somatic embryogenesis were selected as FCF-tolerant. Alternaria carthami fungal spores (5 × 105 spores/ml) sprayed on the leaves of FCF-tolerant plants showed enhanced survival rate over control plants, which plants were more susceptible to fungal attack. The number of leaf spot lesions per leaf was decreased from 3.4 to 0.9 and their lesion length was also reduced from 2.9 to 0.7 mm in organogenic derived FCF-tolerant plants over control. In somatic embryo derived FCF-tolerant plants, the number of lesions was decreased from 3.1 to 0.4 and the lesion size was also reduced to 2.7-0.5 mm when compared to the control. This study also examined antioxidant enzyme activity in FCF-tolerant plants. Catalase (CAT) activity was slightly decreased whereas peroxidase (POD) activity was increased to a maximum of 42% (0.19 μmol min-1mg-1 protein) from organogenesis and 47% (0.23 μmol min-1mg-1 protein) from embryogenesis in FCF-tolerant plants. Superoxide dismutase (SOD) activity was also increased to 17% (149 U mg-1 protein) and 19.5% (145 U mg-1 protein) in FCF-tolerant plants derived from organogenesis and somatic embryogenesis when compared with control plants.

Idioma originalInglés
Páginas (desde-hasta)85-96
Número de páginas12
PublicaciónPlant Cell, Tissue and Organ Culture
Volumen93
N.º1
DOI
EstadoPublicada - 1 abr 2008

Huella dactilar

Alternaria carthami
Carthamus tinctorius
culture filtrates
organogenesis
somatic embryogenesis
lesions (plant)
callus
embryo (plant)
microbial culture
plantlets
proteins
leaf spot

Citar esto

@article{fecb4411756e427db188735680a6d2cd,
title = "Production of plants resistant to Alternaria carthami via organogenesis and somatic embryogenesis of safflower cv. NARI-6 treated with fungal culture filtrates",
abstract = "The present study describes a system for efficient plant regeneration via organogenesis and somatic embryogenesis of safflower (Carthamus tinctorius L.) cv. NARI-6 in fungal culture filtrates (FCF)-treated cultures. FCF was prepared by culturing Alternaria carthami fungal mycelia in selection medium for host-specific toxin production. Cotyledon explants cultured on callus induction medium with different levels of FCF (10-50{\%}) produced embryogenic callus. In organogenesis, 42.2{\%} microshoots formed directly from embryogenic callus tissues in plant regeneration medium with 40{\%} FCF. Isolated embryogenic callus cultured on embryo induction medium containing 40{\%} FCF induced 50.2{\%} somatic embryogenesis. Embryo germination percentage was decreased from 64.5 to 28 in embryo maturation medium containing 40{\%} FCF. However, nine plantlets from organogenesis and 24 plantlets from somatic embryogenesis were selected as FCF-tolerant. Alternaria carthami fungal spores (5 × 105 spores/ml) sprayed on the leaves of FCF-tolerant plants showed enhanced survival rate over control plants, which plants were more susceptible to fungal attack. The number of leaf spot lesions per leaf was decreased from 3.4 to 0.9 and their lesion length was also reduced from 2.9 to 0.7 mm in organogenic derived FCF-tolerant plants over control. In somatic embryo derived FCF-tolerant plants, the number of lesions was decreased from 3.1 to 0.4 and the lesion size was also reduced to 2.7-0.5 mm when compared to the control. This study also examined antioxidant enzyme activity in FCF-tolerant plants. Catalase (CAT) activity was slightly decreased whereas peroxidase (POD) activity was increased to a maximum of 42{\%} (0.19 μmol min-1mg-1 protein) from organogenesis and 47{\%} (0.23 μmol min-1mg-1 protein) from embryogenesis in FCF-tolerant plants. Superoxide dismutase (SOD) activity was also increased to 17{\%} (149 U mg-1 protein) and 19.5{\%} (145 U mg-1 protein) in FCF-tolerant plants derived from organogenesis and somatic embryogenesis when compared with control plants.",
keywords = "Alternaria leaf spot, Antioxidant enzymes, Carthamus tinctorius L., Disease resistance, Embryogenic calli, Medicinal plant, Organogenesis, Plant regeneration, Somatic embryogenesis",
author = "{Vijaya Kumar}, J. and {Ranjitha Kumari}, {B. D.} and G. Sujatha and {Casta{\~n}o De la serna}, Enrique",
year = "2008",
month = "4",
day = "1",
doi = "10.1007/s11240-008-9346-4",
language = "Ingl{\'e}s",
volume = "93",
pages = "85--96",
journal = "Plant Cell, Tissue and Organ Culture",
issn = "0167-6857",
publisher = "Springer Netherlands",
number = "1",

}

Production of plants resistant to Alternaria carthami via organogenesis and somatic embryogenesis of safflower cv. NARI-6 treated with fungal culture filtrates. / Vijaya Kumar, J.; Ranjitha Kumari, B. D.; Sujatha, G.; Castaño De la serna, Enrique.

En: Plant Cell, Tissue and Organ Culture, Vol. 93, N.º 1, 01.04.2008, p. 85-96.

Resultado de la investigación: Contribución a una revistaArtículo

TY - JOUR

T1 - Production of plants resistant to Alternaria carthami via organogenesis and somatic embryogenesis of safflower cv. NARI-6 treated with fungal culture filtrates

AU - Vijaya Kumar, J.

AU - Ranjitha Kumari, B. D.

AU - Sujatha, G.

AU - Castaño De la serna, Enrique

PY - 2008/4/1

Y1 - 2008/4/1

N2 - The present study describes a system for efficient plant regeneration via organogenesis and somatic embryogenesis of safflower (Carthamus tinctorius L.) cv. NARI-6 in fungal culture filtrates (FCF)-treated cultures. FCF was prepared by culturing Alternaria carthami fungal mycelia in selection medium for host-specific toxin production. Cotyledon explants cultured on callus induction medium with different levels of FCF (10-50%) produced embryogenic callus. In organogenesis, 42.2% microshoots formed directly from embryogenic callus tissues in plant regeneration medium with 40% FCF. Isolated embryogenic callus cultured on embryo induction medium containing 40% FCF induced 50.2% somatic embryogenesis. Embryo germination percentage was decreased from 64.5 to 28 in embryo maturation medium containing 40% FCF. However, nine plantlets from organogenesis and 24 plantlets from somatic embryogenesis were selected as FCF-tolerant. Alternaria carthami fungal spores (5 × 105 spores/ml) sprayed on the leaves of FCF-tolerant plants showed enhanced survival rate over control plants, which plants were more susceptible to fungal attack. The number of leaf spot lesions per leaf was decreased from 3.4 to 0.9 and their lesion length was also reduced from 2.9 to 0.7 mm in organogenic derived FCF-tolerant plants over control. In somatic embryo derived FCF-tolerant plants, the number of lesions was decreased from 3.1 to 0.4 and the lesion size was also reduced to 2.7-0.5 mm when compared to the control. This study also examined antioxidant enzyme activity in FCF-tolerant plants. Catalase (CAT) activity was slightly decreased whereas peroxidase (POD) activity was increased to a maximum of 42% (0.19 μmol min-1mg-1 protein) from organogenesis and 47% (0.23 μmol min-1mg-1 protein) from embryogenesis in FCF-tolerant plants. Superoxide dismutase (SOD) activity was also increased to 17% (149 U mg-1 protein) and 19.5% (145 U mg-1 protein) in FCF-tolerant plants derived from organogenesis and somatic embryogenesis when compared with control plants.

AB - The present study describes a system for efficient plant regeneration via organogenesis and somatic embryogenesis of safflower (Carthamus tinctorius L.) cv. NARI-6 in fungal culture filtrates (FCF)-treated cultures. FCF was prepared by culturing Alternaria carthami fungal mycelia in selection medium for host-specific toxin production. Cotyledon explants cultured on callus induction medium with different levels of FCF (10-50%) produced embryogenic callus. In organogenesis, 42.2% microshoots formed directly from embryogenic callus tissues in plant regeneration medium with 40% FCF. Isolated embryogenic callus cultured on embryo induction medium containing 40% FCF induced 50.2% somatic embryogenesis. Embryo germination percentage was decreased from 64.5 to 28 in embryo maturation medium containing 40% FCF. However, nine plantlets from organogenesis and 24 plantlets from somatic embryogenesis were selected as FCF-tolerant. Alternaria carthami fungal spores (5 × 105 spores/ml) sprayed on the leaves of FCF-tolerant plants showed enhanced survival rate over control plants, which plants were more susceptible to fungal attack. The number of leaf spot lesions per leaf was decreased from 3.4 to 0.9 and their lesion length was also reduced from 2.9 to 0.7 mm in organogenic derived FCF-tolerant plants over control. In somatic embryo derived FCF-tolerant plants, the number of lesions was decreased from 3.1 to 0.4 and the lesion size was also reduced to 2.7-0.5 mm when compared to the control. This study also examined antioxidant enzyme activity in FCF-tolerant plants. Catalase (CAT) activity was slightly decreased whereas peroxidase (POD) activity was increased to a maximum of 42% (0.19 μmol min-1mg-1 protein) from organogenesis and 47% (0.23 μmol min-1mg-1 protein) from embryogenesis in FCF-tolerant plants. Superoxide dismutase (SOD) activity was also increased to 17% (149 U mg-1 protein) and 19.5% (145 U mg-1 protein) in FCF-tolerant plants derived from organogenesis and somatic embryogenesis when compared with control plants.

KW - Alternaria leaf spot

KW - Antioxidant enzymes

KW - Carthamus tinctorius L.

KW - Disease resistance

KW - Embryogenic calli

KW - Medicinal plant

KW - Organogenesis

KW - Plant regeneration

KW - Somatic embryogenesis

UR - http://www.scopus.com/inward/record.url?scp=41049101968&partnerID=8YFLogxK

U2 - 10.1007/s11240-008-9346-4

DO - 10.1007/s11240-008-9346-4

M3 - Artículo

AN - SCOPUS:41049101968

VL - 93

SP - 85

EP - 96

JO - Plant Cell, Tissue and Organ Culture

JF - Plant Cell, Tissue and Organ Culture

SN - 0167-6857

IS - 1

ER -