M3 muscarinic receptors mediate cholinergic excitation of the spontaneous activity of subthalamic neurons in the rat

Gonzalo Flores, Salvador Hernández, Manuel G. Rosales, Arturo Sierra, Daniel Martínes-Fong, Jorge Flores-Hernández, Jorge Aceves

Resultado de la investigación: Contribución a una revistaArtículo

26 Citas (Scopus)

Resumen

The effect of the muscarinic antagonist on carbachol-induced increase in spontaneous activity of neurons of the subthalamic nucleus was examined by recording the extracellular unitary activity in an in vitro slice preparation. Carbachol produced (98% of the 263 neurons tested) an increase (twofold of the basal at 500 nM) of the discharge frequency. The EC50 for the carbachol-induced effect was 375 ± 8.7 nM (mean ± SEM). The response was blocked by muscarinic antagonists in a dose dependent manner. However, the IC50 (94 ± 3 nM) for the Mg antagonist 4-diphenyl acetoxy-N-methyl piperidine methobromide (4-DAMP) was considerably less than the other muscarinic antagonists (M1 antagonist pirenzepine, IC50 1340 ± 110 nM; M2 antagonist AF-DX-116, IC50 6780 ± 690 nM). These results suggest that the cholinergic input to the rat subthalamic nucleus exerts a postsynaptic excitatory action and this effect is likely mediated via muscarinic receptor type 3.

Idioma originalInglés
Páginas (desde-hasta)203-206
Número de páginas4
PublicaciónNeuroscience Letters
Volumen203
N.º3
DOI
EstadoPublicada - 26 ene 1996

Huella dactilar

Muscarinic M3 Receptors
Muscarinic Antagonists
Carbachol
Cholinergic Agents
Inhibitory Concentration 50
Subthalamic Nucleus
Neurons
Pirenzepine
Muscarinic Receptors

Citar esto

Flores, G., Hernández, S., Rosales, M. G., Sierra, A., Martínes-Fong, D., Flores-Hernández, J., & Aceves, J. (1996). M3 muscarinic receptors mediate cholinergic excitation of the spontaneous activity of subthalamic neurons in the rat. Neuroscience Letters, 203(3), 203-206. https://doi.org/10.1016/0304-3940(95)12297-4
Flores, Gonzalo ; Hernández, Salvador ; Rosales, Manuel G. ; Sierra, Arturo ; Martínes-Fong, Daniel ; Flores-Hernández, Jorge ; Aceves, Jorge. / M3 muscarinic receptors mediate cholinergic excitation of the spontaneous activity of subthalamic neurons in the rat. En: Neuroscience Letters. 1996 ; Vol. 203, N.º 3. pp. 203-206.
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abstract = "The effect of the muscarinic antagonist on carbachol-induced increase in spontaneous activity of neurons of the subthalamic nucleus was examined by recording the extracellular unitary activity in an in vitro slice preparation. Carbachol produced (98{\%} of the 263 neurons tested) an increase (twofold of the basal at 500 nM) of the discharge frequency. The EC50 for the carbachol-induced effect was 375 ± 8.7 nM (mean ± SEM). The response was blocked by muscarinic antagonists in a dose dependent manner. However, the IC50 (94 ± 3 nM) for the Mg antagonist 4-diphenyl acetoxy-N-methyl piperidine methobromide (4-DAMP) was considerably less than the other muscarinic antagonists (M1 antagonist pirenzepine, IC50 1340 ± 110 nM; M2 antagonist AF-DX-116, IC50 6780 ± 690 nM). These results suggest that the cholinergic input to the rat subthalamic nucleus exerts a postsynaptic excitatory action and this effect is likely mediated via muscarinic receptor type 3.",
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Flores, G, Hernández, S, Rosales, MG, Sierra, A, Martínes-Fong, D, Flores-Hernández, J & Aceves, J 1996, 'M3 muscarinic receptors mediate cholinergic excitation of the spontaneous activity of subthalamic neurons in the rat', Neuroscience Letters, vol. 203, n.º 3, pp. 203-206. https://doi.org/10.1016/0304-3940(95)12297-4

M3 muscarinic receptors mediate cholinergic excitation of the spontaneous activity of subthalamic neurons in the rat. / Flores, Gonzalo; Hernández, Salvador; Rosales, Manuel G.; Sierra, Arturo; Martínes-Fong, Daniel; Flores-Hernández, Jorge; Aceves, Jorge.

En: Neuroscience Letters, Vol. 203, N.º 3, 26.01.1996, p. 203-206.

Resultado de la investigación: Contribución a una revistaArtículo

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T1 - M3 muscarinic receptors mediate cholinergic excitation of the spontaneous activity of subthalamic neurons in the rat

AU - Flores, Gonzalo

AU - Hernández, Salvador

AU - Rosales, Manuel G.

AU - Sierra, Arturo

AU - Martínes-Fong, Daniel

AU - Flores-Hernández, Jorge

AU - Aceves, Jorge

PY - 1996/1/26

Y1 - 1996/1/26

N2 - The effect of the muscarinic antagonist on carbachol-induced increase in spontaneous activity of neurons of the subthalamic nucleus was examined by recording the extracellular unitary activity in an in vitro slice preparation. Carbachol produced (98% of the 263 neurons tested) an increase (twofold of the basal at 500 nM) of the discharge frequency. The EC50 for the carbachol-induced effect was 375 ± 8.7 nM (mean ± SEM). The response was blocked by muscarinic antagonists in a dose dependent manner. However, the IC50 (94 ± 3 nM) for the Mg antagonist 4-diphenyl acetoxy-N-methyl piperidine methobromide (4-DAMP) was considerably less than the other muscarinic antagonists (M1 antagonist pirenzepine, IC50 1340 ± 110 nM; M2 antagonist AF-DX-116, IC50 6780 ± 690 nM). These results suggest that the cholinergic input to the rat subthalamic nucleus exerts a postsynaptic excitatory action and this effect is likely mediated via muscarinic receptor type 3.

AB - The effect of the muscarinic antagonist on carbachol-induced increase in spontaneous activity of neurons of the subthalamic nucleus was examined by recording the extracellular unitary activity in an in vitro slice preparation. Carbachol produced (98% of the 263 neurons tested) an increase (twofold of the basal at 500 nM) of the discharge frequency. The EC50 for the carbachol-induced effect was 375 ± 8.7 nM (mean ± SEM). The response was blocked by muscarinic antagonists in a dose dependent manner. However, the IC50 (94 ± 3 nM) for the Mg antagonist 4-diphenyl acetoxy-N-methyl piperidine methobromide (4-DAMP) was considerably less than the other muscarinic antagonists (M1 antagonist pirenzepine, IC50 1340 ± 110 nM; M2 antagonist AF-DX-116, IC50 6780 ± 690 nM). These results suggest that the cholinergic input to the rat subthalamic nucleus exerts a postsynaptic excitatory action and this effect is likely mediated via muscarinic receptor type 3.

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KW - Basal ganglia

KW - Carbachol

KW - Cholinergic action

KW - Pirenzepine

KW - Subthalamic nucleus

KW - Trihexyphenidyl

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