Hydrogen production by a mixed photoheterotrophic culture: Correlation between gene expression analysis and physiological behavior

V. H. Jurado-Marban, M. A. Tapia-Bustos, R. A. Gonzalez-Garcia, E. Salgado-Manjarrez, Elvia Ines Garcia Peña

Resultado de la investigación: Contribución a una revistaArtículo

1 Cita (Scopus)

Resumen

Photoheterotrophic bacteria use mechanisms such as H 2 production, carbon dioxide fixation, and the polyhydroxybutyrate (PHB) synthesis to maintain redox balance. Therefore, these aspects must be studied from a metabolic and genomic perspective to optimize the H 2 production. In this study, six genes (nifH, hupS, hoxA, nuoF, phbC and depA) in a photoheterotrophic mixed culture, whose predominant population was Rhodopseudomonas palustris, were studied. The transcriptional activity of the genes was determined by quantitative-PCR under two different conditions: H 2 production and PHB accumulation. Differences in the genes expression level were found compared to those reported in pure strains. The high expression of the nuoF gene during H 2 production, suggest that this is a key enzyme to regulate the reducing power, and it was correlated with a high H 2 yield (3.4 mol H 2 /mol acetate). This last aspect is also explained by the low expression of the uptake hydrogenase gene, hupS.

Idioma originalInglés
Páginas (desde-hasta)641-651
Número de páginas11
PublicaciónInternational Journal of Hydrogen Energy
Volumen44
N.º2
DOI
EstadoPublicada - 8 ene 2019

Huella dactilar

gene expression
hydrogen production
Hydrogen production
Gene expression
genes
Genes
bacteria
carbon dioxide
enzymes
acetates
Carbon dioxide
Bacteria
Enzymes
synthesis

Citar esto

Jurado-Marban, V. H. ; Tapia-Bustos, M. A. ; Gonzalez-Garcia, R. A. ; Salgado-Manjarrez, E. ; Garcia Peña, Elvia Ines. / Hydrogen production by a mixed photoheterotrophic culture : Correlation between gene expression analysis and physiological behavior. En: International Journal of Hydrogen Energy. 2019 ; Vol. 44, N.º 2. pp. 641-651.
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abstract = "Photoheterotrophic bacteria use mechanisms such as H 2 production, carbon dioxide fixation, and the polyhydroxybutyrate (PHB) synthesis to maintain redox balance. Therefore, these aspects must be studied from a metabolic and genomic perspective to optimize the H 2 production. In this study, six genes (nifH, hupS, hoxA, nuoF, phbC and depA) in a photoheterotrophic mixed culture, whose predominant population was Rhodopseudomonas palustris, were studied. The transcriptional activity of the genes was determined by quantitative-PCR under two different conditions: H 2 production and PHB accumulation. Differences in the genes expression level were found compared to those reported in pure strains. The high expression of the nuoF gene during H 2 production, suggest that this is a key enzyme to regulate the reducing power, and it was correlated with a high H 2 yield (3.4 mol H 2 /mol acetate). This last aspect is also explained by the low expression of the uptake hydrogenase gene, hupS.",
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Hydrogen production by a mixed photoheterotrophic culture : Correlation between gene expression analysis and physiological behavior. / Jurado-Marban, V. H.; Tapia-Bustos, M. A.; Gonzalez-Garcia, R. A.; Salgado-Manjarrez, E.; Garcia Peña, Elvia Ines.

En: International Journal of Hydrogen Energy, Vol. 44, N.º 2, 08.01.2019, p. 641-651.

Resultado de la investigación: Contribución a una revistaArtículo

TY - JOUR

T1 - Hydrogen production by a mixed photoheterotrophic culture

T2 - Correlation between gene expression analysis and physiological behavior

AU - Jurado-Marban, V. H.

AU - Tapia-Bustos, M. A.

AU - Gonzalez-Garcia, R. A.

AU - Salgado-Manjarrez, E.

AU - Garcia Peña, Elvia Ines

PY - 2019/1/8

Y1 - 2019/1/8

N2 - Photoheterotrophic bacteria use mechanisms such as H 2 production, carbon dioxide fixation, and the polyhydroxybutyrate (PHB) synthesis to maintain redox balance. Therefore, these aspects must be studied from a metabolic and genomic perspective to optimize the H 2 production. In this study, six genes (nifH, hupS, hoxA, nuoF, phbC and depA) in a photoheterotrophic mixed culture, whose predominant population was Rhodopseudomonas palustris, were studied. The transcriptional activity of the genes was determined by quantitative-PCR under two different conditions: H 2 production and PHB accumulation. Differences in the genes expression level were found compared to those reported in pure strains. The high expression of the nuoF gene during H 2 production, suggest that this is a key enzyme to regulate the reducing power, and it was correlated with a high H 2 yield (3.4 mol H 2 /mol acetate). This last aspect is also explained by the low expression of the uptake hydrogenase gene, hupS.

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KW - Hydrogenase uptake gene (hupS)

KW - NADH ubiquinone dehydrogenase gene (nuoF)

KW - Nitrogenase gene (nifH)

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