First report of Neofusicoccum algeriense causing dieback of red raspberry in Mexico

R. E. Serret-López, B. Tlapal-Bolaños, S. G. Leyva-Mir, K. C. Correia, F. Méndez-Jaimes, J. M. Tovar-Pedraza, M. Camacho-Tapia

Resultado de la investigación: Contribución a una revistaArtículo

Resumen

During 2014 and 2015, severe symptoms of dieback were observed in a commercial orchard of red raspberry (Rubus idaeus L.) cv. Heritage in Tangancícuaro, Michoacán, Mexico. Symptoms consisted of brown cankers and dieback on canes, accompanied by reddening and wilting of foliage, at an incidence of 5%. Internal symptoms included a brown discoloration of vascular tissues. A total of 30 stem pieces (∼20 cm in length) were collected from 15 diseased plants. Sixty colonies grown on potato dextrose agar (PDA) at 25°C developed a white, aerial mycelium that became olivaceous gray at the surface after 5 days. Pycnidia were produced after 2 to 3 weeks on autoclaved pine needles placed on water agar and were solitary or aggregated, dark brown to black, uniloculate, and 320 to 417 μm in diameter. Conidiophores were hyaline, cylindrical, smooth, thin walled, and septate. Conidia were hyaline, fusiform, base subtruncate to bluntly rounded, smooth, thin walled, aseptate, and (13-) 16 to 19 (-21) × (4-) 4.5 to 5.5 (-6) μm (n = 100). These morphological characters were consistent with the description of Neofusicoccum algeriense reported by Berraf-Tebbal et al. (2014). The isolate was deposited in the Culture Collection of Phytopathogenic Fungi of the Department of Agricultural Parasitology at the Chapingo Autonomous University under accession number UACH-138. For accurate identification of this isolate, internal transcribed spacer region (ITS), β-tubulin gene (BT2), and the translation elongation factor 1-α gene (EF1-α) were amplified using universal primers ITS5/ITS4 (White et al. 1990), Bt2a/Bt2b (Glass and Donaldson 1995), and EF1-728F/EF1-986R (Carbone and Kohn 1999), and sequenced. BLAST queries of the sequences shared 99% identity with the ex-type isolate of N. algeriense for BT2 (KX505915) and EF1-α (KJ657715), and 100% with Neofusicoccum sp. for ITS (KX505905). Additionally, maximum likelihood analysis and Bayesian inference using multilocus alignment (ITS, BT2, and EF1-α) placed the isolate in the same clade with ex-type isolate of N. algeriense. Sequences were deposited as GenBank accession nos. KY609176 (ITS), KY609175 (BT2), and KY609174 (EF1-α). The pathogenicity of the isolate of N. algeriense was confirmed on 25 6-month-old red raspberry plants (cv. Heritage). Canes (70 cm long) were wounded (1.5 cm long, 2 mm deep) at 30 cm above ground level using a scalpel. A colonized 8-mm agar plug from a 7-day-old colony growing on PDA was placed on every wound and wrapped with Parafilm. Five red raspberry control plants received only noncolonized PDA plugs. All plants were covered with plastic bags for 72 h and were maintained in a greenhouse at 25°C. The experiment was repeated twice. After 20 days, all inoculated canes developed cankers, internal necrosis of wood, and dieback. No symptoms were observed on the control plants. The pathogen was reisolated from 100% of the inoculated stems, and microscopic examination showed that it was identical morphologically to that originally observed. To our knowledge, this is the first report of N. algeriense causing dieback of red raspberry in Mexico. This pathogen has been reported causing grapevine trunk disease in Algeria (Berraf-Tebbal et al. 2014), and associated with Eucalyptus globulus plantations in Portugal (Barradas et al. 2016). This disease is an emerging threat to red raspberry production in Mexico and must be taken into consideration in future studies on effective management practices.

Idioma originalInglés
Número de páginas1
PublicaciónPlant Disease
Volumen101
N.º9
DOI
EstadoPublicada - sep 2017

Huella dactilar

Neofusicoccum
Rubus idaeus
dieback
Mexico
agar
signs and symptoms (plants)
internal transcribed spacers
canes
cankers (plants)
genes
potatoes
glucose
scalpels
pycnidia
parasitology
probability analysis
conidiophores
conifer needles
stems
plastic bags

Citar esto

Serret-López, R. E., Tlapal-Bolaños, B., Leyva-Mir, S. G., Correia, K. C., Méndez-Jaimes, F., Tovar-Pedraza, J. M., & Camacho-Tapia, M. (2017). First report of Neofusicoccum algeriense causing dieback of red raspberry in Mexico. Plant Disease, 101(9). https://doi.org/10.1094/PDIS-02-17-0251-PDN
Serret-López, R. E. ; Tlapal-Bolaños, B. ; Leyva-Mir, S. G. ; Correia, K. C. ; Méndez-Jaimes, F. ; Tovar-Pedraza, J. M. ; Camacho-Tapia, M. / First report of Neofusicoccum algeriense causing dieback of red raspberry in Mexico. En: Plant Disease. 2017 ; Vol. 101, N.º 9.
@article{90d7b5c3d090491b99aa84a4cbeefe98,
title = "First report of Neofusicoccum algeriense causing dieback of red raspberry in Mexico",
abstract = "During 2014 and 2015, severe symptoms of dieback were observed in a commercial orchard of red raspberry (Rubus idaeus L.) cv. Heritage in Tanganc{\'i}cuaro, Michoac{\'a}n, Mexico. Symptoms consisted of brown cankers and dieback on canes, accompanied by reddening and wilting of foliage, at an incidence of 5{\%}. Internal symptoms included a brown discoloration of vascular tissues. A total of 30 stem pieces (∼20 cm in length) were collected from 15 diseased plants. Sixty colonies grown on potato dextrose agar (PDA) at 25°C developed a white, aerial mycelium that became olivaceous gray at the surface after 5 days. Pycnidia were produced after 2 to 3 weeks on autoclaved pine needles placed on water agar and were solitary or aggregated, dark brown to black, uniloculate, and 320 to 417 μm in diameter. Conidiophores were hyaline, cylindrical, smooth, thin walled, and septate. Conidia were hyaline, fusiform, base subtruncate to bluntly rounded, smooth, thin walled, aseptate, and (13-) 16 to 19 (-21) × (4-) 4.5 to 5.5 (-6) μm (n = 100). These morphological characters were consistent with the description of Neofusicoccum algeriense reported by Berraf-Tebbal et al. (2014). The isolate was deposited in the Culture Collection of Phytopathogenic Fungi of the Department of Agricultural Parasitology at the Chapingo Autonomous University under accession number UACH-138. For accurate identification of this isolate, internal transcribed spacer region (ITS), β-tubulin gene (BT2), and the translation elongation factor 1-α gene (EF1-α) were amplified using universal primers ITS5/ITS4 (White et al. 1990), Bt2a/Bt2b (Glass and Donaldson 1995), and EF1-728F/EF1-986R (Carbone and Kohn 1999), and sequenced. BLAST queries of the sequences shared 99{\%} identity with the ex-type isolate of N. algeriense for BT2 (KX505915) and EF1-α (KJ657715), and 100{\%} with Neofusicoccum sp. for ITS (KX505905). Additionally, maximum likelihood analysis and Bayesian inference using multilocus alignment (ITS, BT2, and EF1-α) placed the isolate in the same clade with ex-type isolate of N. algeriense. Sequences were deposited as GenBank accession nos. KY609176 (ITS), KY609175 (BT2), and KY609174 (EF1-α). The pathogenicity of the isolate of N. algeriense was confirmed on 25 6-month-old red raspberry plants (cv. Heritage). Canes (70 cm long) were wounded (1.5 cm long, 2 mm deep) at 30 cm above ground level using a scalpel. A colonized 8-mm agar plug from a 7-day-old colony growing on PDA was placed on every wound and wrapped with Parafilm. Five red raspberry control plants received only noncolonized PDA plugs. All plants were covered with plastic bags for 72 h and were maintained in a greenhouse at 25°C. The experiment was repeated twice. After 20 days, all inoculated canes developed cankers, internal necrosis of wood, and dieback. No symptoms were observed on the control plants. The pathogen was reisolated from 100{\%} of the inoculated stems, and microscopic examination showed that it was identical morphologically to that originally observed. To our knowledge, this is the first report of N. algeriense causing dieback of red raspberry in Mexico. This pathogen has been reported causing grapevine trunk disease in Algeria (Berraf-Tebbal et al. 2014), and associated with Eucalyptus globulus plantations in Portugal (Barradas et al. 2016). This disease is an emerging threat to red raspberry production in Mexico and must be taken into consideration in future studies on effective management practices.",
author = "Serret-L{\'o}pez, {R. E.} and B. Tlapal-Bola{\~n}os and Leyva-Mir, {S. G.} and Correia, {K. C.} and F. M{\'e}ndez-Jaimes and Tovar-Pedraza, {J. M.} and M. Camacho-Tapia",
year = "2017",
month = "9",
doi = "10.1094/PDIS-02-17-0251-PDN",
language = "Ingl{\'e}s",
volume = "101",
journal = "Plant Disease",
issn = "0191-2917",
publisher = "American Phytopathological Society",
number = "9",

}

Serret-López, RE, Tlapal-Bolaños, B, Leyva-Mir, SG, Correia, KC, Méndez-Jaimes, F, Tovar-Pedraza, JM & Camacho-Tapia, M 2017, 'First report of Neofusicoccum algeriense causing dieback of red raspberry in Mexico', Plant Disease, vol. 101, n.º 9. https://doi.org/10.1094/PDIS-02-17-0251-PDN

First report of Neofusicoccum algeriense causing dieback of red raspberry in Mexico. / Serret-López, R. E.; Tlapal-Bolaños, B.; Leyva-Mir, S. G.; Correia, K. C.; Méndez-Jaimes, F.; Tovar-Pedraza, J. M.; Camacho-Tapia, M.

En: Plant Disease, Vol. 101, N.º 9, 09.2017.

Resultado de la investigación: Contribución a una revistaArtículo

TY - JOUR

T1 - First report of Neofusicoccum algeriense causing dieback of red raspberry in Mexico

AU - Serret-López, R. E.

AU - Tlapal-Bolaños, B.

AU - Leyva-Mir, S. G.

AU - Correia, K. C.

AU - Méndez-Jaimes, F.

AU - Tovar-Pedraza, J. M.

AU - Camacho-Tapia, M.

PY - 2017/9

Y1 - 2017/9

N2 - During 2014 and 2015, severe symptoms of dieback were observed in a commercial orchard of red raspberry (Rubus idaeus L.) cv. Heritage in Tangancícuaro, Michoacán, Mexico. Symptoms consisted of brown cankers and dieback on canes, accompanied by reddening and wilting of foliage, at an incidence of 5%. Internal symptoms included a brown discoloration of vascular tissues. A total of 30 stem pieces (∼20 cm in length) were collected from 15 diseased plants. Sixty colonies grown on potato dextrose agar (PDA) at 25°C developed a white, aerial mycelium that became olivaceous gray at the surface after 5 days. Pycnidia were produced after 2 to 3 weeks on autoclaved pine needles placed on water agar and were solitary or aggregated, dark brown to black, uniloculate, and 320 to 417 μm in diameter. Conidiophores were hyaline, cylindrical, smooth, thin walled, and septate. Conidia were hyaline, fusiform, base subtruncate to bluntly rounded, smooth, thin walled, aseptate, and (13-) 16 to 19 (-21) × (4-) 4.5 to 5.5 (-6) μm (n = 100). These morphological characters were consistent with the description of Neofusicoccum algeriense reported by Berraf-Tebbal et al. (2014). The isolate was deposited in the Culture Collection of Phytopathogenic Fungi of the Department of Agricultural Parasitology at the Chapingo Autonomous University under accession number UACH-138. For accurate identification of this isolate, internal transcribed spacer region (ITS), β-tubulin gene (BT2), and the translation elongation factor 1-α gene (EF1-α) were amplified using universal primers ITS5/ITS4 (White et al. 1990), Bt2a/Bt2b (Glass and Donaldson 1995), and EF1-728F/EF1-986R (Carbone and Kohn 1999), and sequenced. BLAST queries of the sequences shared 99% identity with the ex-type isolate of N. algeriense for BT2 (KX505915) and EF1-α (KJ657715), and 100% with Neofusicoccum sp. for ITS (KX505905). Additionally, maximum likelihood analysis and Bayesian inference using multilocus alignment (ITS, BT2, and EF1-α) placed the isolate in the same clade with ex-type isolate of N. algeriense. Sequences were deposited as GenBank accession nos. KY609176 (ITS), KY609175 (BT2), and KY609174 (EF1-α). The pathogenicity of the isolate of N. algeriense was confirmed on 25 6-month-old red raspberry plants (cv. Heritage). Canes (70 cm long) were wounded (1.5 cm long, 2 mm deep) at 30 cm above ground level using a scalpel. A colonized 8-mm agar plug from a 7-day-old colony growing on PDA was placed on every wound and wrapped with Parafilm. Five red raspberry control plants received only noncolonized PDA plugs. All plants were covered with plastic bags for 72 h and were maintained in a greenhouse at 25°C. The experiment was repeated twice. After 20 days, all inoculated canes developed cankers, internal necrosis of wood, and dieback. No symptoms were observed on the control plants. The pathogen was reisolated from 100% of the inoculated stems, and microscopic examination showed that it was identical morphologically to that originally observed. To our knowledge, this is the first report of N. algeriense causing dieback of red raspberry in Mexico. This pathogen has been reported causing grapevine trunk disease in Algeria (Berraf-Tebbal et al. 2014), and associated with Eucalyptus globulus plantations in Portugal (Barradas et al. 2016). This disease is an emerging threat to red raspberry production in Mexico and must be taken into consideration in future studies on effective management practices.

AB - During 2014 and 2015, severe symptoms of dieback were observed in a commercial orchard of red raspberry (Rubus idaeus L.) cv. Heritage in Tangancícuaro, Michoacán, Mexico. Symptoms consisted of brown cankers and dieback on canes, accompanied by reddening and wilting of foliage, at an incidence of 5%. Internal symptoms included a brown discoloration of vascular tissues. A total of 30 stem pieces (∼20 cm in length) were collected from 15 diseased plants. Sixty colonies grown on potato dextrose agar (PDA) at 25°C developed a white, aerial mycelium that became olivaceous gray at the surface after 5 days. Pycnidia were produced after 2 to 3 weeks on autoclaved pine needles placed on water agar and were solitary or aggregated, dark brown to black, uniloculate, and 320 to 417 μm in diameter. Conidiophores were hyaline, cylindrical, smooth, thin walled, and septate. Conidia were hyaline, fusiform, base subtruncate to bluntly rounded, smooth, thin walled, aseptate, and (13-) 16 to 19 (-21) × (4-) 4.5 to 5.5 (-6) μm (n = 100). These morphological characters were consistent with the description of Neofusicoccum algeriense reported by Berraf-Tebbal et al. (2014). The isolate was deposited in the Culture Collection of Phytopathogenic Fungi of the Department of Agricultural Parasitology at the Chapingo Autonomous University under accession number UACH-138. For accurate identification of this isolate, internal transcribed spacer region (ITS), β-tubulin gene (BT2), and the translation elongation factor 1-α gene (EF1-α) were amplified using universal primers ITS5/ITS4 (White et al. 1990), Bt2a/Bt2b (Glass and Donaldson 1995), and EF1-728F/EF1-986R (Carbone and Kohn 1999), and sequenced. BLAST queries of the sequences shared 99% identity with the ex-type isolate of N. algeriense for BT2 (KX505915) and EF1-α (KJ657715), and 100% with Neofusicoccum sp. for ITS (KX505905). Additionally, maximum likelihood analysis and Bayesian inference using multilocus alignment (ITS, BT2, and EF1-α) placed the isolate in the same clade with ex-type isolate of N. algeriense. Sequences were deposited as GenBank accession nos. KY609176 (ITS), KY609175 (BT2), and KY609174 (EF1-α). The pathogenicity of the isolate of N. algeriense was confirmed on 25 6-month-old red raspberry plants (cv. Heritage). Canes (70 cm long) were wounded (1.5 cm long, 2 mm deep) at 30 cm above ground level using a scalpel. A colonized 8-mm agar plug from a 7-day-old colony growing on PDA was placed on every wound and wrapped with Parafilm. Five red raspberry control plants received only noncolonized PDA plugs. All plants were covered with plastic bags for 72 h and were maintained in a greenhouse at 25°C. The experiment was repeated twice. After 20 days, all inoculated canes developed cankers, internal necrosis of wood, and dieback. No symptoms were observed on the control plants. The pathogen was reisolated from 100% of the inoculated stems, and microscopic examination showed that it was identical morphologically to that originally observed. To our knowledge, this is the first report of N. algeriense causing dieback of red raspberry in Mexico. This pathogen has been reported causing grapevine trunk disease in Algeria (Berraf-Tebbal et al. 2014), and associated with Eucalyptus globulus plantations in Portugal (Barradas et al. 2016). This disease is an emerging threat to red raspberry production in Mexico and must be taken into consideration in future studies on effective management practices.

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DO - 10.1094/PDIS-02-17-0251-PDN

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AN - SCOPUS:85027572693

VL - 101

JO - Plant Disease

JF - Plant Disease

SN - 0191-2917

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Serret-López RE, Tlapal-Bolaños B, Leyva-Mir SG, Correia KC, Méndez-Jaimes F, Tovar-Pedraza JM y otros. First report of Neofusicoccum algeriense causing dieback of red raspberry in Mexico. Plant Disease. 2017 sep;101(9). https://doi.org/10.1094/PDIS-02-17-0251-PDN