Evaluation of magnetic nanoprobe for breast cancer immunolabeling: Experimental study in vivo

J. G. Silva, J. L. Cruz, G. C. Villanueva, N. M. Perez, L. Huerta, A. V. Vargas, S. Soto, R. Soto, C. A. Gonzalez

Resultado de la investigación: Capítulo del libro/informe/acta de congresoContribución a la conferencia

Resumen

Currently, application of nanotechnology in biomedicine contributes in progress related to the diagnosis and treatment of breast cancer. Our research group is working on the development of an innovative technology to promote the selective ablation of breast cancer in its early stages by the use of high frequency magnetic fields assisted by magnetic nanoparticles. This study is focused to show experimentally the pass of a nanoprobe “Magnetic nanoparticle-antibody anti cerbB-2” from the vascular endothelium to the neoplastic epithelium. A nanoprobe was developed by the covalent union of fluorescent magnetic nanoparticles and anti cerbB-2 Mab by carbodiimide method. A breast cancer model induced in rats with N-methyl, N-nitrosourea (MNU) was employed. Systemic infusion of nanoprobe was conducted and guided to the cancerous tissue by an external magnet positioned in the tumor region. Dissection and histopathological characterization of tumor as well as the detection of nanoprobe by an immunofluorescence assay were performed. The results indicate that the induced tumor corresponds to an invasive ductal carcinoma, and immunofluorescence analysis showed the presence of the nanoprobe in tumoral ducts, which is indicative of the direct path of the nanoprobe into the tumor and its binding to c-erbB-2 expressed in the cytoplasmic membrane of tumor cells.

Idioma originalInglés
Título de la publicación alojadaVI Latin American Congress on Biomedical Engineering, CLAIB 2014
EditoresAriel Braidot, Alejandro Hadad
EditorialSpringer Verlag
Páginas113-116
Número de páginas4
ISBN (versión digital)9783319131160
DOI
EstadoPublicada - 1 ene 2015
Evento6th Latin American Congress on Biomedical Engineering, CLAIB 2014 - Paraná, Argentina
Duración: 29 oct 201431 oct 2014

Serie de la publicación

NombreIFMBE Proceedings
Volumen49
ISSN (versión impresa)1680-0737

Otros

Otros6th Latin American Congress on Biomedical Engineering, CLAIB 2014
PaísArgentina
CiudadParaná
Período29/10/1431/10/14

Huella dactilar

Nanoprobes
Tumors
Nanoparticles
Methylnitrosourea
Carbodiimides
Dissection
Ablation
Nanotechnology
Antibodies
Ducts
Magnets
Rats
Anti-Idiotypic Antibodies
Assays
Cells
Tissue
Magnetic fields
Membranes

Citar esto

Silva, J. G., Cruz, J. L., Villanueva, G. C., Perez, N. M., Huerta, L., Vargas, A. V., ... Gonzalez, C. A. (2015). Evaluation of magnetic nanoprobe for breast cancer immunolabeling: Experimental study in vivo. En A. Braidot, & A. Hadad (Eds.), VI Latin American Congress on Biomedical Engineering, CLAIB 2014 (pp. 113-116). (IFMBE Proceedings; Vol. 49). Springer Verlag. https://doi.org/10.1007/978-3-319-13117-7_30
Silva, J. G. ; Cruz, J. L. ; Villanueva, G. C. ; Perez, N. M. ; Huerta, L. ; Vargas, A. V. ; Soto, S. ; Soto, R. ; Gonzalez, C. A. / Evaluation of magnetic nanoprobe for breast cancer immunolabeling : Experimental study in vivo. VI Latin American Congress on Biomedical Engineering, CLAIB 2014. editor / Ariel Braidot ; Alejandro Hadad. Springer Verlag, 2015. pp. 113-116 (IFMBE Proceedings).
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title = "Evaluation of magnetic nanoprobe for breast cancer immunolabeling: Experimental study in vivo",
abstract = "Currently, application of nanotechnology in biomedicine contributes in progress related to the diagnosis and treatment of breast cancer. Our research group is working on the development of an innovative technology to promote the selective ablation of breast cancer in its early stages by the use of high frequency magnetic fields assisted by magnetic nanoparticles. This study is focused to show experimentally the pass of a nanoprobe “Magnetic nanoparticle-antibody anti cerbB-2” from the vascular endothelium to the neoplastic epithelium. A nanoprobe was developed by the covalent union of fluorescent magnetic nanoparticles and anti cerbB-2 Mab by carbodiimide method. A breast cancer model induced in rats with N-methyl, N-nitrosourea (MNU) was employed. Systemic infusion of nanoprobe was conducted and guided to the cancerous tissue by an external magnet positioned in the tumor region. Dissection and histopathological characterization of tumor as well as the detection of nanoprobe by an immunofluorescence assay were performed. The results indicate that the induced tumor corresponds to an invasive ductal carcinoma, and immunofluorescence analysis showed the presence of the nanoprobe in tumoral ducts, which is indicative of the direct path of the nanoprobe into the tumor and its binding to c-erbB-2 expressed in the cytoplasmic membrane of tumor cells.",
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Silva, JG, Cruz, JL, Villanueva, GC, Perez, NM, Huerta, L, Vargas, AV, Soto, S, Soto, R & Gonzalez, CA 2015, Evaluation of magnetic nanoprobe for breast cancer immunolabeling: Experimental study in vivo. En A Braidot & A Hadad (eds.), VI Latin American Congress on Biomedical Engineering, CLAIB 2014. IFMBE Proceedings, vol. 49, Springer Verlag, pp. 113-116, 6th Latin American Congress on Biomedical Engineering, CLAIB 2014, Paraná, Argentina, 29/10/14. https://doi.org/10.1007/978-3-319-13117-7_30

Evaluation of magnetic nanoprobe for breast cancer immunolabeling : Experimental study in vivo. / Silva, J. G.; Cruz, J. L.; Villanueva, G. C.; Perez, N. M.; Huerta, L.; Vargas, A. V.; Soto, S.; Soto, R.; Gonzalez, C. A.

VI Latin American Congress on Biomedical Engineering, CLAIB 2014. ed. / Ariel Braidot; Alejandro Hadad. Springer Verlag, 2015. p. 113-116 (IFMBE Proceedings; Vol. 49).

Resultado de la investigación: Capítulo del libro/informe/acta de congresoContribución a la conferencia

TY - GEN

T1 - Evaluation of magnetic nanoprobe for breast cancer immunolabeling

T2 - Experimental study in vivo

AU - Silva, J. G.

AU - Cruz, J. L.

AU - Villanueva, G. C.

AU - Perez, N. M.

AU - Huerta, L.

AU - Vargas, A. V.

AU - Soto, S.

AU - Soto, R.

AU - Gonzalez, C. A.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Currently, application of nanotechnology in biomedicine contributes in progress related to the diagnosis and treatment of breast cancer. Our research group is working on the development of an innovative technology to promote the selective ablation of breast cancer in its early stages by the use of high frequency magnetic fields assisted by magnetic nanoparticles. This study is focused to show experimentally the pass of a nanoprobe “Magnetic nanoparticle-antibody anti cerbB-2” from the vascular endothelium to the neoplastic epithelium. A nanoprobe was developed by the covalent union of fluorescent magnetic nanoparticles and anti cerbB-2 Mab by carbodiimide method. A breast cancer model induced in rats with N-methyl, N-nitrosourea (MNU) was employed. Systemic infusion of nanoprobe was conducted and guided to the cancerous tissue by an external magnet positioned in the tumor region. Dissection and histopathological characterization of tumor as well as the detection of nanoprobe by an immunofluorescence assay were performed. The results indicate that the induced tumor corresponds to an invasive ductal carcinoma, and immunofluorescence analysis showed the presence of the nanoprobe in tumoral ducts, which is indicative of the direct path of the nanoprobe into the tumor and its binding to c-erbB-2 expressed in the cytoplasmic membrane of tumor cells.

AB - Currently, application of nanotechnology in biomedicine contributes in progress related to the diagnosis and treatment of breast cancer. Our research group is working on the development of an innovative technology to promote the selective ablation of breast cancer in its early stages by the use of high frequency magnetic fields assisted by magnetic nanoparticles. This study is focused to show experimentally the pass of a nanoprobe “Magnetic nanoparticle-antibody anti cerbB-2” from the vascular endothelium to the neoplastic epithelium. A nanoprobe was developed by the covalent union of fluorescent magnetic nanoparticles and anti cerbB-2 Mab by carbodiimide method. A breast cancer model induced in rats with N-methyl, N-nitrosourea (MNU) was employed. Systemic infusion of nanoprobe was conducted and guided to the cancerous tissue by an external magnet positioned in the tumor region. Dissection and histopathological characterization of tumor as well as the detection of nanoprobe by an immunofluorescence assay were performed. The results indicate that the induced tumor corresponds to an invasive ductal carcinoma, and immunofluorescence analysis showed the presence of the nanoprobe in tumoral ducts, which is indicative of the direct path of the nanoprobe into the tumor and its binding to c-erbB-2 expressed in the cytoplasmic membrane of tumor cells.

KW - Breast

KW - Cancer

KW - Magnetic Nanoparticle

KW - Nanoprobe

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M3 - Contribución a la conferencia

AN - SCOPUS:84925270204

T3 - IFMBE Proceedings

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BT - VI Latin American Congress on Biomedical Engineering, CLAIB 2014

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A2 - Hadad, Alejandro

PB - Springer Verlag

ER -

Silva JG, Cruz JL, Villanueva GC, Perez NM, Huerta L, Vargas AV y otros. Evaluation of magnetic nanoprobe for breast cancer immunolabeling: Experimental study in vivo. En Braidot A, Hadad A, editores, VI Latin American Congress on Biomedical Engineering, CLAIB 2014. Springer Verlag. 2015. p. 113-116. (IFMBE Proceedings). https://doi.org/10.1007/978-3-319-13117-7_30