Effect of neutral electrolyzed water on lux-tagged Listeria monocytogenes EGDe biofilms adhered to stainless steel and visualization with destructive and non-destructive microscopy techniques

M. Arevalos-Sánchez, C. Regalado, S. E. Martin, Yunny Meas Vong, E. Cadena-Moreno, B. E. García-Almendárez

Resultado de la investigación: Contribución a una revistaArtículo

21 Citas (Scopus)

Resumen

The presence of Listeria monocytogenes in food processing environment is a risk of food contamination by persistent cells due to their ability to attach to stainless steel and other surfaces. We aimed to study biofilms formation of lux-tagged L.monocytogenes EGDe on stainless steel surfaces and their control using neutral electrolyzed water (NEW), where biofilms development was monitored using destructive and non-destructive microscopy techniques. The development of biofilms was monitored for 5 days on stainless steel chips. We used two sources of NEW, commercial (NEW-1) and from a prototype (NEW-2) for treatments of free and biofilm L.monocytogenes EGDe cells. Complete inhibition of L.monocytogenes EGDe free cells was observed after 1min contact time for both NEW sources, but NEW-1 concentration used (9mg/L total available chlorine, TAC) was 1.8 times higher. Cells within biofilms were more resistant to NEW compared to planktonic cells. Same concentration of both NEW sources (70mg/L TAC) exhibited complete inhibition of biofilm cells after 3min contact time. However, using a sub-lethal dose of 40mg/L TAC, NEW-2 reduced about 2logCFU/cm2 biofilm cells while NEW-1 inhibited 0.3logCFU/cm2 only. Biofilms formation and antagonistic effect of NEW could be visualized by epifluorescence and scanning electron microscopy, revealing significant biofilms structure. The disinfectant effect of NEW may be attributed to the combined antimicrobial effect of available chlorine and high ORP exhibited by its oxidizing compounds. NEW does not promote metal equipment corrosion due to its neutral pH, and is also environmentally friendly.

Idioma originalInglés
Páginas (desde-hasta)472-477
Número de páginas6
PublicaciónFood Control
Volumen34
N.º2
DOI
EstadoPublicada - 1 dic 2013

Huella dactilar

Stainless Steel
Listeria monocytogenes
stainless steel
Biofilms
biofilm
Microscopy
microscopy
Water
Chlorine
chlorine
methodology
cells
electrolyzed water
Food Contamination
Food Handling
Corrosion
Disinfectants
Water Purification
corrosion
disinfectants

Citar esto

Arevalos-Sánchez, M. ; Regalado, C. ; Martin, S. E. ; Meas Vong, Yunny ; Cadena-Moreno, E. ; García-Almendárez, B. E. / Effect of neutral electrolyzed water on lux-tagged Listeria monocytogenes EGDe biofilms adhered to stainless steel and visualization with destructive and non-destructive microscopy techniques. En: Food Control. 2013 ; Vol. 34, N.º 2. pp. 472-477.
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abstract = "The presence of Listeria monocytogenes in food processing environment is a risk of food contamination by persistent cells due to their ability to attach to stainless steel and other surfaces. We aimed to study biofilms formation of lux-tagged L.monocytogenes EGDe on stainless steel surfaces and their control using neutral electrolyzed water (NEW), where biofilms development was monitored using destructive and non-destructive microscopy techniques. The development of biofilms was monitored for 5 days on stainless steel chips. We used two sources of NEW, commercial (NEW-1) and from a prototype (NEW-2) for treatments of free and biofilm L.monocytogenes EGDe cells. Complete inhibition of L.monocytogenes EGDe free cells was observed after 1min contact time for both NEW sources, but NEW-1 concentration used (9mg/L total available chlorine, TAC) was 1.8 times higher. Cells within biofilms were more resistant to NEW compared to planktonic cells. Same concentration of both NEW sources (70mg/L TAC) exhibited complete inhibition of biofilm cells after 3min contact time. However, using a sub-lethal dose of 40mg/L TAC, NEW-2 reduced about 2logCFU/cm2 biofilm cells while NEW-1 inhibited 0.3logCFU/cm2 only. Biofilms formation and antagonistic effect of NEW could be visualized by epifluorescence and scanning electron microscopy, revealing significant biofilms structure. The disinfectant effect of NEW may be attributed to the combined antimicrobial effect of available chlorine and high ORP exhibited by its oxidizing compounds. NEW does not promote metal equipment corrosion due to its neutral pH, and is also environmentally friendly.",
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Effect of neutral electrolyzed water on lux-tagged Listeria monocytogenes EGDe biofilms adhered to stainless steel and visualization with destructive and non-destructive microscopy techniques. / Arevalos-Sánchez, M.; Regalado, C.; Martin, S. E.; Meas Vong, Yunny; Cadena-Moreno, E.; García-Almendárez, B. E.

En: Food Control, Vol. 34, N.º 2, 01.12.2013, p. 472-477.

Resultado de la investigación: Contribución a una revistaArtículo

TY - JOUR

T1 - Effect of neutral electrolyzed water on lux-tagged Listeria monocytogenes EGDe biofilms adhered to stainless steel and visualization with destructive and non-destructive microscopy techniques

AU - Arevalos-Sánchez, M.

AU - Regalado, C.

AU - Martin, S. E.

AU - Meas Vong, Yunny

AU - Cadena-Moreno, E.

AU - García-Almendárez, B. E.

PY - 2013/12/1

Y1 - 2013/12/1

N2 - The presence of Listeria monocytogenes in food processing environment is a risk of food contamination by persistent cells due to their ability to attach to stainless steel and other surfaces. We aimed to study biofilms formation of lux-tagged L.monocytogenes EGDe on stainless steel surfaces and their control using neutral electrolyzed water (NEW), where biofilms development was monitored using destructive and non-destructive microscopy techniques. The development of biofilms was monitored for 5 days on stainless steel chips. We used two sources of NEW, commercial (NEW-1) and from a prototype (NEW-2) for treatments of free and biofilm L.monocytogenes EGDe cells. Complete inhibition of L.monocytogenes EGDe free cells was observed after 1min contact time for both NEW sources, but NEW-1 concentration used (9mg/L total available chlorine, TAC) was 1.8 times higher. Cells within biofilms were more resistant to NEW compared to planktonic cells. Same concentration of both NEW sources (70mg/L TAC) exhibited complete inhibition of biofilm cells after 3min contact time. However, using a sub-lethal dose of 40mg/L TAC, NEW-2 reduced about 2logCFU/cm2 biofilm cells while NEW-1 inhibited 0.3logCFU/cm2 only. Biofilms formation and antagonistic effect of NEW could be visualized by epifluorescence and scanning electron microscopy, revealing significant biofilms structure. The disinfectant effect of NEW may be attributed to the combined antimicrobial effect of available chlorine and high ORP exhibited by its oxidizing compounds. NEW does not promote metal equipment corrosion due to its neutral pH, and is also environmentally friendly.

AB - The presence of Listeria monocytogenes in food processing environment is a risk of food contamination by persistent cells due to their ability to attach to stainless steel and other surfaces. We aimed to study biofilms formation of lux-tagged L.monocytogenes EGDe on stainless steel surfaces and their control using neutral electrolyzed water (NEW), where biofilms development was monitored using destructive and non-destructive microscopy techniques. The development of biofilms was monitored for 5 days on stainless steel chips. We used two sources of NEW, commercial (NEW-1) and from a prototype (NEW-2) for treatments of free and biofilm L.monocytogenes EGDe cells. Complete inhibition of L.monocytogenes EGDe free cells was observed after 1min contact time for both NEW sources, but NEW-1 concentration used (9mg/L total available chlorine, TAC) was 1.8 times higher. Cells within biofilms were more resistant to NEW compared to planktonic cells. Same concentration of both NEW sources (70mg/L TAC) exhibited complete inhibition of biofilm cells after 3min contact time. However, using a sub-lethal dose of 40mg/L TAC, NEW-2 reduced about 2logCFU/cm2 biofilm cells while NEW-1 inhibited 0.3logCFU/cm2 only. Biofilms formation and antagonistic effect of NEW could be visualized by epifluorescence and scanning electron microscopy, revealing significant biofilms structure. The disinfectant effect of NEW may be attributed to the combined antimicrobial effect of available chlorine and high ORP exhibited by its oxidizing compounds. NEW does not promote metal equipment corrosion due to its neutral pH, and is also environmentally friendly.

KW - Biofilms

KW - Epifluorescence

KW - Lux-tagged Listeria monocytogenes

KW - Neutral electrolyzed water

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DO - 10.1016/j.foodcont.2013.05.021

M3 - Artículo

AN - SCOPUS:84879288928

VL - 34

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EP - 477

JO - Food Control

JF - Food Control

SN - 0956-7135

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