DNA barcoding and fauna of phlebotomine sand flies (Diptera: Psychodidae: Phlebotominae) from Los Tuxtlas, Veracruz, Mexico

Yokomi N. Lozano-Sardaneta, Luís E. Paternina, Sokani Sánchez-Montes, Alejandro Quintero, Sergio Ibáñez-Bernal, Víctor Sánchez-Cordero, Eduar Elías Bejarano, Ingeborg Becker

Resultado de la investigación: Contribución a una revistaArtículo

Resumen

Mexico has great diversity of phlebotomine sand flies related to cases of leishmaniasis, yet few studies have dressed the molecular taxonomy of these sand fly species. The use of the cytochrome oxidase subunit 1 (COI) gene, as a DNA Barcode has facilitated the molecular identification of sand flies species worldwide. We use the DNA barcode as a useful tool for the identification of phlebotomine sand flies of the natural reserve Los Tuxtlas from Veracruz, México. A fragment of 536 bp of the COI gene was obtained from 36 individuals belonging to eight species of five genera (Dampfomyia, Lutzomyia, Psathyromyia, Psychodopygus and Brumptomyia) with coverage between 92–100%, and found similarities ranging from 93–98% with other New World phlebotomine sand flies. The NJ dendogram grouped sand flies into eight clusters according to identified species, supported by bootstrap of 97%–100%. In conclusion, all phlebotomine sand flies were correctly identified and agree with the morphological identification, also could separate genetics the isomorphic females of the genus Brumptomyia.

Idioma originalInglés
Número de artículo105220
PublicaciónActa Tropica
Volumen201
DOI
EstadoPublicada - ene 2020

Huella dactilar

Psychodidae
Phlebotominae
DNA barcoding
Mexico
Diptera
fauna
DNA
Electron Transport Complex IV
Lutzomyia
cytochrome-c oxidase
Leishmaniasis
Genes
molecular systematics
leishmaniasis
genes

Citar esto

Lozano-Sardaneta, Y. N., Paternina, L. E., Sánchez-Montes, S., Quintero, A., Ibáñez-Bernal, S., Sánchez-Cordero, V., ... Becker, I. (2020). DNA barcoding and fauna of phlebotomine sand flies (Diptera: Psychodidae: Phlebotominae) from Los Tuxtlas, Veracruz, Mexico. Acta Tropica, 201, [105220]. https://doi.org/10.1016/j.actatropica.2019.105220
Lozano-Sardaneta, Yokomi N. ; Paternina, Luís E. ; Sánchez-Montes, Sokani ; Quintero, Alejandro ; Ibáñez-Bernal, Sergio ; Sánchez-Cordero, Víctor ; Bejarano, Eduar Elías ; Becker, Ingeborg. / DNA barcoding and fauna of phlebotomine sand flies (Diptera : Psychodidae: Phlebotominae) from Los Tuxtlas, Veracruz, Mexico. En: Acta Tropica. 2020 ; Vol. 201.
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title = "DNA barcoding and fauna of phlebotomine sand flies (Diptera: Psychodidae: Phlebotominae) from Los Tuxtlas, Veracruz, Mexico",
abstract = "Mexico has great diversity of phlebotomine sand flies related to cases of leishmaniasis, yet few studies have dressed the molecular taxonomy of these sand fly species. The use of the cytochrome oxidase subunit 1 (COI) gene, as a DNA Barcode has facilitated the molecular identification of sand flies species worldwide. We use the DNA barcode as a useful tool for the identification of phlebotomine sand flies of the natural reserve Los Tuxtlas from Veracruz, M{\'e}xico. A fragment of 536 bp of the COI gene was obtained from 36 individuals belonging to eight species of five genera (Dampfomyia, Lutzomyia, Psathyromyia, Psychodopygus and Brumptomyia) with coverage between 92–100{\%}, and found similarities ranging from 93–98{\%} with other New World phlebotomine sand flies. The NJ dendogram grouped sand flies into eight clusters according to identified species, supported by bootstrap of 97{\%}–100{\%}. In conclusion, all phlebotomine sand flies were correctly identified and agree with the morphological identification, also could separate genetics the isomorphic females of the genus Brumptomyia.",
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Lozano-Sardaneta, YN, Paternina, LE, Sánchez-Montes, S, Quintero, A, Ibáñez-Bernal, S, Sánchez-Cordero, V, Bejarano, EE & Becker, I 2020, 'DNA barcoding and fauna of phlebotomine sand flies (Diptera: Psychodidae: Phlebotominae) from Los Tuxtlas, Veracruz, Mexico', Acta Tropica, vol. 201, 105220. https://doi.org/10.1016/j.actatropica.2019.105220

DNA barcoding and fauna of phlebotomine sand flies (Diptera : Psychodidae: Phlebotominae) from Los Tuxtlas, Veracruz, Mexico. / Lozano-Sardaneta, Yokomi N.; Paternina, Luís E.; Sánchez-Montes, Sokani; Quintero, Alejandro; Ibáñez-Bernal, Sergio; Sánchez-Cordero, Víctor; Bejarano, Eduar Elías; Becker, Ingeborg.

En: Acta Tropica, Vol. 201, 105220, 01.2020.

Resultado de la investigación: Contribución a una revistaArtículo

TY - JOUR

T1 - DNA barcoding and fauna of phlebotomine sand flies (Diptera

T2 - Psychodidae: Phlebotominae) from Los Tuxtlas, Veracruz, Mexico

AU - Lozano-Sardaneta, Yokomi N.

AU - Paternina, Luís E.

AU - Sánchez-Montes, Sokani

AU - Quintero, Alejandro

AU - Ibáñez-Bernal, Sergio

AU - Sánchez-Cordero, Víctor

AU - Bejarano, Eduar Elías

AU - Becker, Ingeborg

PY - 2020/1

Y1 - 2020/1

N2 - Mexico has great diversity of phlebotomine sand flies related to cases of leishmaniasis, yet few studies have dressed the molecular taxonomy of these sand fly species. The use of the cytochrome oxidase subunit 1 (COI) gene, as a DNA Barcode has facilitated the molecular identification of sand flies species worldwide. We use the DNA barcode as a useful tool for the identification of phlebotomine sand flies of the natural reserve Los Tuxtlas from Veracruz, México. A fragment of 536 bp of the COI gene was obtained from 36 individuals belonging to eight species of five genera (Dampfomyia, Lutzomyia, Psathyromyia, Psychodopygus and Brumptomyia) with coverage between 92–100%, and found similarities ranging from 93–98% with other New World phlebotomine sand flies. The NJ dendogram grouped sand flies into eight clusters according to identified species, supported by bootstrap of 97%–100%. In conclusion, all phlebotomine sand flies were correctly identified and agree with the morphological identification, also could separate genetics the isomorphic females of the genus Brumptomyia.

AB - Mexico has great diversity of phlebotomine sand flies related to cases of leishmaniasis, yet few studies have dressed the molecular taxonomy of these sand fly species. The use of the cytochrome oxidase subunit 1 (COI) gene, as a DNA Barcode has facilitated the molecular identification of sand flies species worldwide. We use the DNA barcode as a useful tool for the identification of phlebotomine sand flies of the natural reserve Los Tuxtlas from Veracruz, México. A fragment of 536 bp of the COI gene was obtained from 36 individuals belonging to eight species of five genera (Dampfomyia, Lutzomyia, Psathyromyia, Psychodopygus and Brumptomyia) with coverage between 92–100%, and found similarities ranging from 93–98% with other New World phlebotomine sand flies. The NJ dendogram grouped sand flies into eight clusters according to identified species, supported by bootstrap of 97%–100%. In conclusion, all phlebotomine sand flies were correctly identified and agree with the morphological identification, also could separate genetics the isomorphic females of the genus Brumptomyia.

KW - Brumptomyia

KW - Cytochrome oxidase subunit I

KW - Genetics

KW - Lutzomyia

UR - http://www.scopus.com/inward/record.url?scp=85073670025&partnerID=8YFLogxK

U2 - 10.1016/j.actatropica.2019.105220

DO - 10.1016/j.actatropica.2019.105220

M3 - Artículo

C2 - 31618609

AN - SCOPUS:85073670025

VL - 201

JO - Acta Tropica

JF - Acta Tropica

SN - 0001-706X

M1 - 105220

ER -

Lozano-Sardaneta YN, Paternina LE, Sánchez-Montes S, Quintero A, Ibáñez-Bernal S, Sánchez-Cordero V y otros. DNA barcoding and fauna of phlebotomine sand flies (Diptera: Psychodidae: Phlebotominae) from Los Tuxtlas, Veracruz, Mexico. Acta Tropica. 2020 ene;201. 105220. https://doi.org/10.1016/j.actatropica.2019.105220