Biosprospecting potential of kelp (Laminariales, Phaeophyceae) from Baja California Peninsula: phenolic content, antioxidant properties, anti-inflammatory, and cell viability

Paola A. Tenorio-Rodríguez, Hugo Esquivel-Solis, Jesús I. Murillo-Álvarez, Felipe Ascencio, Ángel I. Campa-Córdova, Carlos Angulo

Resultado de la investigación: Contribución a una revistaArtículo

Resumen

To explore the potential of two kelps as natural sources of bioactive compounds, this study evaluated total phenolic content, antioxidant properties, anti-inflammatory, and cell viability activities of polyphenol-enriched fractions from Eisenia arborea and Macrocystis pyrifera. Overall, the fractions from E. arborea had higher antioxidant properties than M. pyrifera. However, two fractions from E. arborea and M. pyrifera showed the strongest antioxidant activities among 12 fractions tested by polyphenol content, 1,1-diphenyl-2-picryl-hydrazyl (DPPH), and Ferric reducing antioxidant power (FRAP) assays. Several fractions upregulated IL-10 gene expression and downregulated TNF-α and iNOS mRNA in RAW 264.7 cells. Remarkably, most of the fractions at the highest concentration (1280 μg mL−1) showed non-toxic effect on L9S29 cell line. Analysis of fractions by Fourier transformed infrared from attenuated total reflectance (FTIR-ATR) and by ultra-performance liquid chromatography coupled to electrospray ionization quadrupole time of flight mass spectrometry (UPLC-ESI-Q-TOF-MS) suggested the presence of phlorotannins, such as phloroeckol, fucophloroethol, and phlorofucofuroeckol. In conclusion, the polyphenol content (phlorotannins) varied among fractions of macroalgal species and influencing their biological properties, which represent natural sources for health promotion.

Idioma originalInglés
Páginas (desde-hasta)3115-3129
Número de páginas15
PublicaciónJournal of Applied Phycology
Volumen31
N.º5
DOI
EstadoPublicada - 1 oct 2019

Huella dactilar

Eisenia arborea
Laminariales
Macrocystis pyrifera
Phaeophyceae
anti-inflammatory activity
antioxidant
cell viability
macroalgae
polyphenols
viability
antioxidants
ultra-performance liquid chromatography
health promotion
interleukin-10
ionization
reflectance
antioxidant activity
cell lines
mass spectrometry
gene expression

Citar esto

Tenorio-Rodríguez, Paola A. ; Esquivel-Solis, Hugo ; Murillo-Álvarez, Jesús I. ; Ascencio, Felipe ; Campa-Córdova, Ángel I. ; Angulo, Carlos. / Biosprospecting potential of kelp (Laminariales, Phaeophyceae) from Baja California Peninsula : phenolic content, antioxidant properties, anti-inflammatory, and cell viability. En: Journal of Applied Phycology. 2019 ; Vol. 31, N.º 5. pp. 3115-3129.
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abstract = "To explore the potential of two kelps as natural sources of bioactive compounds, this study evaluated total phenolic content, antioxidant properties, anti-inflammatory, and cell viability activities of polyphenol-enriched fractions from Eisenia arborea and Macrocystis pyrifera. Overall, the fractions from E. arborea had higher antioxidant properties than M. pyrifera. However, two fractions from E. arborea and M. pyrifera showed the strongest antioxidant activities among 12 fractions tested by polyphenol content, 1,1-diphenyl-2-picryl-hydrazyl (DPPH), and Ferric reducing antioxidant power (FRAP) assays. Several fractions upregulated IL-10 gene expression and downregulated TNF-α and iNOS mRNA in RAW 264.7 cells. Remarkably, most of the fractions at the highest concentration (1280 μg mL−1) showed non-toxic effect on L9S29 cell line. Analysis of fractions by Fourier transformed infrared from attenuated total reflectance (FTIR-ATR) and by ultra-performance liquid chromatography coupled to electrospray ionization quadrupole time of flight mass spectrometry (UPLC-ESI-Q-TOF-MS) suggested the presence of phlorotannins, such as phloroeckol, fucophloroethol, and phlorofucofuroeckol. In conclusion, the polyphenol content (phlorotannins) varied among fractions of macroalgal species and influencing their biological properties, which represent natural sources for health promotion.",
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Biosprospecting potential of kelp (Laminariales, Phaeophyceae) from Baja California Peninsula : phenolic content, antioxidant properties, anti-inflammatory, and cell viability. / Tenorio-Rodríguez, Paola A.; Esquivel-Solis, Hugo; Murillo-Álvarez, Jesús I.; Ascencio, Felipe; Campa-Córdova, Ángel I.; Angulo, Carlos.

En: Journal of Applied Phycology, Vol. 31, N.º 5, 01.10.2019, p. 3115-3129.

Resultado de la investigación: Contribución a una revistaArtículo

TY - JOUR

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T2 - phenolic content, antioxidant properties, anti-inflammatory, and cell viability

AU - Tenorio-Rodríguez, Paola A.

AU - Esquivel-Solis, Hugo

AU - Murillo-Álvarez, Jesús I.

AU - Ascencio, Felipe

AU - Campa-Córdova, Ángel I.

AU - Angulo, Carlos

PY - 2019/10/1

Y1 - 2019/10/1

N2 - To explore the potential of two kelps as natural sources of bioactive compounds, this study evaluated total phenolic content, antioxidant properties, anti-inflammatory, and cell viability activities of polyphenol-enriched fractions from Eisenia arborea and Macrocystis pyrifera. Overall, the fractions from E. arborea had higher antioxidant properties than M. pyrifera. However, two fractions from E. arborea and M. pyrifera showed the strongest antioxidant activities among 12 fractions tested by polyphenol content, 1,1-diphenyl-2-picryl-hydrazyl (DPPH), and Ferric reducing antioxidant power (FRAP) assays. Several fractions upregulated IL-10 gene expression and downregulated TNF-α and iNOS mRNA in RAW 264.7 cells. Remarkably, most of the fractions at the highest concentration (1280 μg mL−1) showed non-toxic effect on L9S29 cell line. Analysis of fractions by Fourier transformed infrared from attenuated total reflectance (FTIR-ATR) and by ultra-performance liquid chromatography coupled to electrospray ionization quadrupole time of flight mass spectrometry (UPLC-ESI-Q-TOF-MS) suggested the presence of phlorotannins, such as phloroeckol, fucophloroethol, and phlorofucofuroeckol. In conclusion, the polyphenol content (phlorotannins) varied among fractions of macroalgal species and influencing their biological properties, which represent natural sources for health promotion.

AB - To explore the potential of two kelps as natural sources of bioactive compounds, this study evaluated total phenolic content, antioxidant properties, anti-inflammatory, and cell viability activities of polyphenol-enriched fractions from Eisenia arborea and Macrocystis pyrifera. Overall, the fractions from E. arborea had higher antioxidant properties than M. pyrifera. However, two fractions from E. arborea and M. pyrifera showed the strongest antioxidant activities among 12 fractions tested by polyphenol content, 1,1-diphenyl-2-picryl-hydrazyl (DPPH), and Ferric reducing antioxidant power (FRAP) assays. Several fractions upregulated IL-10 gene expression and downregulated TNF-α and iNOS mRNA in RAW 264.7 cells. Remarkably, most of the fractions at the highest concentration (1280 μg mL−1) showed non-toxic effect on L9S29 cell line. Analysis of fractions by Fourier transformed infrared from attenuated total reflectance (FTIR-ATR) and by ultra-performance liquid chromatography coupled to electrospray ionization quadrupole time of flight mass spectrometry (UPLC-ESI-Q-TOF-MS) suggested the presence of phlorotannins, such as phloroeckol, fucophloroethol, and phlorofucofuroeckol. In conclusion, the polyphenol content (phlorotannins) varied among fractions of macroalgal species and influencing their biological properties, which represent natural sources for health promotion.

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