Antigenic characterization of Plasmodium vivax with monoclonal antibodies

M. R. Sanchez, J. A. Ramirez, J. Larriva-Sahd, M. H. Rodriguez, R. Mancilla, L. Ortiz-Ortiz

Resultado de la investigación: Contribución a una revistaArtículo

4 Citas (Scopus)

Resumen

Monoclonal antibodies were produced against Plasmodium vivax obtained from patients living in southeastern Mexico, where P. vivax malaria is endemic. Nine hybridomas specific for this parasite were obtained. By an indirect immunofluorescence assay, seven antibodies were found to react with epitopes present in the cytoplasm of the infected erythrocyte and two with the parasite itself. By immunoblotting, five monoclonal antibodies reacted with a 17-kD protein band, three with an 85-kD band, and two with one of 45 kD. By immunogold electron microscopy, two antibodies that reacted with the cytoplasm of infected erythrocytes by immunofluorescence also labeled cytoplasmic clefts, and one, in addition, recognized caveola-vesicle complexes and the parasite matrix. These results demonstrate the value of monoclonal antibodies in identifying P. vivax antigens and disclosing their subcellular distribution.

Idioma originalInglés
Páginas (desde-hasta)60-67
Número de páginas8
PublicaciónAmerican Journal of Tropical Medicine and Hygiene
Volumen51
N.º1
DOI
EstadoPublicada - 1 ene 1994

Huella dactilar

Plasmodium vivax
Parasites
Monoclonal Antibodies
Cytoplasm
Erythrocytes
Vivax Malaria
Caveolae
Antibodies
Hybridomas
Indirect Fluorescent Antibody Technique
Mexico
Immunoblotting
Fluorescent Antibody Technique
Epitopes
Electron Microscopy
Antigens
Proteins

Citar esto

Sanchez, M. R. ; Ramirez, J. A. ; Larriva-Sahd, J. ; Rodriguez, M. H. ; Mancilla, R. ; Ortiz-Ortiz, L. / Antigenic characterization of Plasmodium vivax with monoclonal antibodies. En: American Journal of Tropical Medicine and Hygiene. 1994 ; Vol. 51, N.º 1. pp. 60-67.
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Antigenic characterization of Plasmodium vivax with monoclonal antibodies. / Sanchez, M. R.; Ramirez, J. A.; Larriva-Sahd, J.; Rodriguez, M. H.; Mancilla, R.; Ortiz-Ortiz, L.

En: American Journal of Tropical Medicine and Hygiene, Vol. 51, N.º 1, 01.01.1994, p. 60-67.

Resultado de la investigación: Contribución a una revistaArtículo

TY - JOUR

T1 - Antigenic characterization of Plasmodium vivax with monoclonal antibodies

AU - Sanchez, M. R.

AU - Ramirez, J. A.

AU - Larriva-Sahd, J.

AU - Rodriguez, M. H.

AU - Mancilla, R.

AU - Ortiz-Ortiz, L.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Monoclonal antibodies were produced against Plasmodium vivax obtained from patients living in southeastern Mexico, where P. vivax malaria is endemic. Nine hybridomas specific for this parasite were obtained. By an indirect immunofluorescence assay, seven antibodies were found to react with epitopes present in the cytoplasm of the infected erythrocyte and two with the parasite itself. By immunoblotting, five monoclonal antibodies reacted with a 17-kD protein band, three with an 85-kD band, and two with one of 45 kD. By immunogold electron microscopy, two antibodies that reacted with the cytoplasm of infected erythrocytes by immunofluorescence also labeled cytoplasmic clefts, and one, in addition, recognized caveola-vesicle complexes and the parasite matrix. These results demonstrate the value of monoclonal antibodies in identifying P. vivax antigens and disclosing their subcellular distribution.

AB - Monoclonal antibodies were produced against Plasmodium vivax obtained from patients living in southeastern Mexico, where P. vivax malaria is endemic. Nine hybridomas specific for this parasite were obtained. By an indirect immunofluorescence assay, seven antibodies were found to react with epitopes present in the cytoplasm of the infected erythrocyte and two with the parasite itself. By immunoblotting, five monoclonal antibodies reacted with a 17-kD protein band, three with an 85-kD band, and two with one of 45 kD. By immunogold electron microscopy, two antibodies that reacted with the cytoplasm of infected erythrocytes by immunofluorescence also labeled cytoplasmic clefts, and one, in addition, recognized caveola-vesicle complexes and the parasite matrix. These results demonstrate the value of monoclonal antibodies in identifying P. vivax antigens and disclosing their subcellular distribution.

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JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

SN - 0002-9637

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